Animations
The animations provided on this website are copyrighted by Kinexus Bioinformatics Corporation, but permission is given for their free use for personal and teaching purposes. Many of these animations were produced in collaboration with Cameron Bowyer (www.carbondigital.ca). They can be viewed using Quicktime Player. Any inquiries about these animations should be directed to Dr. Steven Pelech at Kinexus Bioinformatics Corporation (Phone: 604.323.2547 ext.10; e-mail: spelech@kinexus.ca)
Animations are categorized under the following sections. To navigate to a category, click on any of the category shortcuts listed below.
Animations are categorized under the following sections. To navigate to a category, click on any of the category shortcuts listed below.
Signal Transduction Proteins in Multimeric Complexes – Space Filling Models
These animations feature rotating 3D x-ray crystallographic structures of representative signalling proteins in complexes, which have been separately colourized to facilitate their visualization.
Download a full legend to protein chain colorization and detailed references for all the signal transduction proteins shown in animations immediately below (ADOBE PDF FORMAT)
Download a full legend to protein chain colorization and detailed references for all the signal transduction proteins shown in animations immediately below (ADOBE PDF FORMAT)
1A02: Nfat, Fos and Jun DNA-binding domains bound to DNA
1A0N: Fyn tyrosine kinase SH3 domain complexed with a synthetic peptide P2L corresponding to residues 91-104 of the p85 subunit of phosphatidylinositol 3-kinase
1A37: 14-3-3 bound to Raf kinase Ser-259 phosphorylation site containing peptide
1A6Q: Protein-serine/threonine phosphatase 2C (PP2C)
1AMP: cAMP-dependent protein kinase (PKA) catalytic subunit complexed with inhibitor portion of PKI inhibitor
1AXC: Proliferating cell nuclear antigen (PCNA) binding to p21 WAF CDK inhibitor
1B17: Insulin A and B chains in a complex
1BUH: Cyclin-dependent kinase 2 (CDK2) complex with cell cycle-regulatory protein Ckshs1
1CS4: Adenylate Cyclase C1A and C2A domains complexed with Gs alpha subunit
1CVS: Fibroblast growth factor 2 (FGF2) dimer - fibroblast growth factor receptor1 (FGFR1) complex
1CXZ: RhoA complexed with the effector domain of the protein kinase PKN/PRK1
1D0G: Death receptor 5 (DR5) extracellular domain bound to Apo2 ligand/Trail
1E0A: Cdc42 complexed with the GTPase binding domain of p21-activated kinase (PAK)
1E0O: Fibroblast growth factor 1 (FGF1) dimer-fibroblast growth factor receptor 2 (FGFR2) complex
1EBA: Erythropoietin (Epo) receptor extracellular domain bound to Epo
1EFN: HIV-1 Nef protein in complex with Arg-96I mutant Fyn SH3 Domain
1ES7: Bone morphogenetic protein (BMP) receptor Ia extracellular domain bound to BMP-2
1F1J: Caspase-7 in complex with Acetyl-Asp-Glu-Val-Asp-Cho
1FAP: FKBP12-rapamycin complex interacting with PI3K-related kinase FRAP fragment
1FPR: SHP-1 tyrosine phosphatase catalytic domain and an in vitro peptide substrate Py469 derived from SHPS-1
1FQ1: Cyclin-dependent kinase 2 (CDK2) complexed with KAP
1G1F: Protein tyrosine phosphatase 1B (PTP1B) complexed with a tri-phosphorylated peptide (Rdi(Ptr) Etd(Ptr)(Ptr)Rk) from the insulin receptor kinase
1G3N: p18(Ink4C)-cyclin-dependent kinase 6 - cyclin K ternary complex
1G6R: Superagonist T cell antigen receptor (TCR)/major histochemistry complex (MHC)
1GNG: Glycogen synthase kinase-3 (GSK3) complex with Frattide peptide
1GOT: Trimeric G protein complex of alpha, beta and gamma subunits
1H10: Pleckstrin homology domain of Protein Kinase B/Akt bound to Ins(1,3,4,5) - tetrakisphosphate
1H90: Phosphatidylinositol 3-kinase (PI3K), p85-subunit: C-terminal SH2 domain complexed with a Tyr-751 phosphorylation site containing peptide from the platelet-derived growth factor receptor
1HE8: Ras G12V - Phosphatidylinositol 3-kinase (PI3K) complex
1I51: Caspase-7 in complex with Xenopus inhibitor of apoptosis (XIAP)
1IRU: Mammalian 20S proteasome complex
1ITB: Interleukin-1 (IL1) type-1 receptor complexed with interleukin-1 beta
1JSU: Cyclin-dependent kinase 2 (CDK2)/cyclin A complexed with p27 KIP1
1JWH: Casein kinase 2 (CK2) holoenzyme alpha and beta subunit quaternary complex
1JSU: Cyclin-dependent kinase 2 (CDK2)/cyclin A complexed with p27 KIP1
1KX3: Histones H2A, H2B, H3 and H4 with DNA in nucleosomes core complexes
1KZO: Protein farnesyltransferase complexed with farnesylated K - Ras4B peptide product and farnesyl diphosphate substrate bound simultaneously
1LAR: LAR receptor tyrosine phosphatase tandem phosphatase domains
1MF8: Calcineurin (PP2B)-calmodulin-cyclophilin-cyclosporin complex
1N8Z: ErbB2 (HER2) receptor tyrosine kinase extracellular domain complexed with herceptin monoclonal antibody Fab fragment
1NT9: RNA polymerase 2 complex
1NVV: Ras-GTP of the Ras-specific nucleotide exchange factor Sos
1O6K: Protein kinase B/Akt kinase domain S474D complexed with glycogen synthase kinase 3 peptide and AMP-PnP
1OMW: G protein coupled receptor kinase 2 (GRK2/BARK) complexed with G-beta/gamma subunits
1P9M: Interleukin-6 (IL6) alpha/interleukin-6 beta receptorand interleukin-6
1PNX: 30S Ribosome RNA and protein complex
1PNY: 50S Ribosome RNA and protein complex
1Q4K: Polo-like kinase 1 (PLK1) polo-box domain in a complex with a phospho-peptide
1RQQ: Insulin receptor tyrosine kinase in a complex with the SH2 domain of adapter APS and a substrate inhibitor
1SHC: Shc PTB domain complexed with a phosphopeptide from the TrkA receptor-tyrosine kinase
1TUP: Tumor suppressor p53 complexed with DNA
1WQ1: Ras complexed with Ras-GTPase activating protein (GAP)
1WWW: Nerve growth factor in complex with domain 5 of the TrkA receptor-tyrosine kinase
2NGR: Cdc42 bound to the active and catalytically compromised forms of the Cdc42-GTPase activating protein (GAP)
CaMK2: Calmodulin-dependent kinase 2 oligomer complexed with calmodulin (artistic composite - CaMK2 catalytic domain represented with phosphorylase kinase catalytic domain)
EGFR+PM: Epidermal growth factor (EGF) receptor-tyrosine kinase domain dimer shown with plasma membrane and extracellular EGF domain (artistic composite)
Signal Transduction Proteins – Ribbon, Stick and Ball 3D Models
These animations feature rotating 3D x-ray crystallographic structures of cell signalling proteins with ribbon, stick and ball representation to facilitate their visualization.
Download a full legend to protein chain colorization and detailed references for all the signal transduction proteins shown in animations immediately below (ADOBE PDF FORMAT)
Download a full legend to protein chain colorization and detailed references for all the signal transduction proteins shown in animations immediately below (ADOBE PDF FORMAT)
1B39: Cyclin-dependent kinase 2 (CDK2) with Thr-160 phosphorylation site - ball model
1D5R: PTEN phosphoinositide phosphatase - ribbon model
1DM2: CDK2 complexed marine sponge inhibitor hymenialdisine - ball model
1E0A: Cdc42 complexed with the GTPase binding domain of p21-activated kinase-alpha (PAKa) - stick model
1E8Z: Phosphatidylinositol 3-kinase gamma (PI3Kg) p110 catalytic subunit bound with inhibitor staurosporin - ribbon
1EBA: Erythropoietin (Epo) receptor extracellular domain bound to Epo - ball model
1F3M: p21-activated kinase-alpha (PAKa) autoregulatory domaincomplexed with its catalytic domain - ball and ribbon model
1FMK: Src protein-tyrosine kinase with SH2, SH3, catalytic domain and C-terminus - wire model
1FMK: Src protein-tyrosine kinase - ribbon model
1FYN: Fyn protein-tyrosine kinase SH3 domain with proline-containing polypeptide from 3BP-2 - ball model
1GRI: Growth factor bound protein 2 (Grb2) - ribbon model
1IAN: p38 MAPK alpha - ball model
1IR3: Insulin receptor-tyrosine kinase catalytic domain with peptide substrate - stick model
1MCO: Immunoglobulin - ball model
1NTS: DNA double helix - stick model
1PTU: Protein-tyrosine phosphatase 1B complexed with a phosphotyrosine-containing hexapeptide - stick model
1QB0: Cdc25B dual specificty phosphatase - ribbon model
1QB3: Cyclin-dependent kinase regulatory subunit (Cks1) - ball model
1QJA: 14-3-3 zeta dimer complexed with Raf1 phosphorylated serine-containing peptide - stick and ball model
1QMZ: Cyclin-dependent kinase 2 (CDK2) complexed with cyclin A - ribbon and ball model
1RVD: H-Ras small G protein G12V constitutively active mutant - ribbon model
1WQ1: Ras complexed with Ras-GTPase activating protein (GAP) - ribbon model
1ZEH: Insulin - ball model
2CSN: Casein kinase 1 (CK1) catalytic subunit - ribbon model
2HNP: Protein-tyrosine phosphatase 1B - ribbon model
4ERK: Extracellular-regulated kinase 2 (ERK2) complexed with drug - ribbon model
4Q21: H-Ras small G protein - inactive form bound to GDP - ribbon model
Conserved Amino Acids in Catalytic Domains of Signal Transduction Proteins
These animations feature 3D x-ray crystallographic structures of representative signalling proteins that have been colourized to show key conserved amino acid residues that form part of the active sites of these enzymes. These animations were produced by Cameron Bowyer and are provided in a version in which the proteins automatically rotate. Please contact Kinexus if you would like to obtain QuickTime VR versions of these animations that allow viewers to manually rotate proteins in any direction with a mouse.
Download legend to key amino acid colorations for all the signal transduction proteins shown immediately below in 3D animations of their rotating x-ray crystallographic structures (ADOBE PDF FORMAT)
Download legend to key amino acid colorations for all the signal transduction proteins shown immediately below in 3D animations of their rotating x-ray crystallographic structures (ADOBE PDF FORMAT)
Signalling Protein Reaction Pathways
These cartoon animations illustrate concepts in protein reactions in cell signalling pathways by showing examples with representative proteins.
