Welcome to the Kinexus Web Site
Celebrating Over a Decade of Innovation and Exploration
Over 1600 laboratories in over 35 countries have benefited from our highly integrated platform of proteomics and bioinformatics services. Our unique services are convenient, cost-effective, insightful and enabling for identification and validation of biomarkers, drug targets and therapeutic compounds. Put our discovery engine to work for you.
What's New
We have made several recent enhancements to our unique Kinex™ Antibody Microarray Services. Here are just nine of the reasons why you should try this powerful and affordable discovery strategy now.
- Convenience - Just send us your frozen biological specimens by courier.
- High Sensitivity - No sample enrichment required. Uses 100 µg of crude cell or tissue lysate protein.
- Wide Coverage - Tracks hundreds of cell signalling proteins with over 800 in-house validated, commercial antibodies (~540 pan-specific and ~270 phosphosite-specific).
- Reduced False Positives – Proprietary chemical cleavage of sample proteins lowers the risk of false positives from protein-protein interactions.
- Improved Surface Chemistry and Printing - With capture antibodies now printed with better morphology and consistency on 3D matrix-coated glass slides and improved signal-to-noise ratios (a dynamic signal detection range over 3 orders of magnitude).
- Single Dye, Non-competitive Methodology - Two samples are labelled with the same dye and incubated separately on the same slide to eliminate differential protein-dye binding problems.
- High Reproducibility - Duplicate measurements provided in detailed MS-Excel reports with further data analyses. Median error range of ~8%.
- Inexpensive Validation – Service available to confirm top18 hits from antibody microarray by immunoblotting. Additional enabling, follow up services are also available.
- Well Tested and Proven - Over 4000 antibody microarray analyses performed to date. Open access to full experimental data from over 2000 analyses available online for comparisons. Over 70 scientific peer-reviewed papers published using our antibody microarrays.
Our free online TranscriptoNET KnowledgeBase was generated from over 125 million measurements of the expression of over 21,000 human genes monitored with over 6000 gene microarrays deposited in the NCBI GEO database. TranscriptoNET can be queried to identify the most commonly, uniquely and highly expressed genes in over 300 human organs, tissues and cells as well as 300 established human tumour cell lines. It identifies those genes that are the most up-regulated and down-regulated in over 50 types of human cancers. It can also be used to identify the co-expression of genes, and similarities in gene expression between organs, tissues and cells.
Our free online PhosphoNET KnowledgeBase features detailed information on nearly 93,000 known and over 560,000 predicted phosphorylation sites in over 23,000 human proteins, including their evolutionary analysis in over 20 other species. Our proprietary Kinase Predictor algorithm has also identified the top 10% of 492 human protein kinases that best match each of these known and putative human phosphosites in PhosphoNET.
Kinexus has expanded our custom In Silico Prediction services to permit unparalleled analysis of protein kinase specificity and protein phosphosite identification. Our In Silico Kinase Specificity Prediction (IKSP) Service predicts the optimal substrate specificity of kinases from humans and many other eukaryotic species using our proprietary algorithms. We can provide a listing of the top 100 candidate human protein kinases that are predicted to phosphorylate any phosphorylation site (including those in other species) with our new In Silico Kinase Match Prediction (IKMP) Services. We can also start with a human protein kinase of interest and identify the top 1000 or 5000 predicted human phosphosites that are likely to be targeted by that kinase. Our new In Silico Phosphoprotein Match Prediction (IPMP) Services predict phosphosites in a target human protein, determine their evolutionary conservation and score the ability of 500 kinases to target each phosphosite. Many of these predictions can be tested in vitro with purified kinases and peptide substrates by Kinexus with our new In Vitro Kinase and Phosphopeptide Testing (IKPT) Service
Kinexus has expanded our custom In Silico Prediction services to permit unparalleled analysis of protein kinase specificity and protein phosphosite identification. Our In Silico Kinase Specificity Prediction (IKSP) Service predicts the optimal substrate specificity of kinases from humans and many other eukaryotic species using our proprietary algorithms. We can provide a listing of the top 100 candidate human protein kinases that are predicted to phosphorylate any phosphorylation site (including those in other species) with our new In Silico Kinase Match Prediction (IKMP) Services. We can also start with a human protein kinase of interest and identify the top 1000 or 5000 predicted human phosphosites that are likely to be targeted by that kinase. Our new In Silico Phosphoprotein Match Prediction (IPMP) Services predict phosphosites in a target human protein, determine their evolutionary conservation and score the ability of 500 kinases to target each phosphosite. Many of these predictions can be tested in vitro with purified kinases and peptide substrates by Kinexus with our new In Vitro Kinase and Phosphopeptide Testing (IKPT) Service
Our unique proteomics services have permitted detection of many antibody cross-reactive proteins that demonstrate profound changes in expression and phosphorylation in response to diverse stimuli in experimental model systems. Our new Protein Identification by Mass Spectrometry (PIMS) Services now lets our clients find out the identities of these interesting proteins. Our PIMS services include the immunoprecipitation of target proteins and LC MS/MS mass spectrometry identification.
While phosphosite antibodies permit the detection of currently about a thousand phosphosites, this is only a fraction of a percent of the actual number of protein phosphorylation sites that exist in cells. Our new Mass Spectrometry Proteome Phosphorylation (MSPP) Services permit the identification of hundreds to thousands of phosphorylation sites that may be differentially regulated in experimental model systems. This powerful methodology provides the most comprehensive analyses available for monitoring the regulation of protein phosphorylation networks. But if you are focused only on the phosphosites located in just one protein, you can use our Mass Spectrometry Protein Phosphosite (MSPS) Profiling Services. If you want to know which proteins and their phosphosites in crude cell lysates are phosphorylated directly by a kinase in vitro then you might be interested in our latest Mass Spectrometry Kinase Substrate (MSKS) Profiling Services.
While phosphosite antibodies permit the detection of currently about a thousand phosphosites, this is only a fraction of a percent of the actual number of protein phosphorylation sites that exist in cells. Our new Mass Spectrometry Proteome Phosphorylation (MSPP) Services permit the identification of hundreds to thousands of phosphorylation sites that may be differentially regulated in experimental model systems. This powerful methodology provides the most comprehensive analyses available for monitoring the regulation of protein phosphorylation networks. But if you are focused only on the phosphosites located in just one protein, you can use our Mass Spectrometry Protein Phosphosite (MSPS) Profiling Services. If you want to know which proteins and their phosphosites in crude cell lysates are phosphorylated directly by a kinase in vitro then you might be interested in our latest Mass Spectrometry Kinase Substrate (MSKS) Profiling Services.
Direct Links to Free Kinexus Databases and Knowledgebases
