IHC Service Options
To maximize the prospects of success, it is critical that we receive as much information about the probing antibody as possible if this is to be supplied by the client. A copy of the Antibody Data Sheet from the commercial vendor should be provided if available. Clients must also fill in the requested information in our Client-Supplied Antibody Description Form IHC-CADF-1.
It is highly recommended that positive control tissues are used for our IHC analyses. In the case of our IHC protocol development service, our experienced staff will carry out a full literature search to find the ideal control tissue(s) to be used for the optimization process. In the case of our standard IHC service, it is up to the client’s discretion to either provide or request for a specific positive control tissue. Following IHC staining, all slides are inspected by our strict Quality Control Unit to ensure all staining criteria are met. Stained slides can then be returned to the client along with digital imaging of the slides.
Preparation of Tissues
If tissue sections are supplied by the client for our IHC Optimization Protocol Service, we will consult with the client whether any potential conflicts may arise from the preparation of the tissue based on the commercial Antibody Data Sheet and the provided Client-Supplied Antibody Description Form IHC-CADF-1. For example, the antibody may only be reactive to tissue sections prepared via frozen (cryo) techniques or specific fixation techniques for proper preservation of the antigen. If tissue sections are supplied by Kinexus, we will use the ideal tissue preparation conditions to achieve optimal IHC staining.
Please note that we cannot guarantee IHC results as the protein(s) of interest may not be present in the tissue(s) of interest. In addition, a positive result from Western blot analysis may not necessarily produce a positive result in IHC due to their inherent differences. In Western blotting, the epitope is readily available in the denatured protein, while IHC may rely on an exposed epitope in the native protein.
If our IHC Optimization Protocol Service is being used with client-supplied slides, a minimum of 20 slides should be sent to Kinexus. At least 5 positive control slides should also be provided. We will return any unused slides provided by our clients unless they indicate otherwise.
To inform us precisely which of the IHC Services that you wish to use from Kinexus, you must complete a Service Order Form (IHC-SOF-01). Filing out this form will provide an estimate of the total costs of the various work that you would like Kinexus to perform and gives us your contact and billing information. You must also complete a Custom Immunohistochemistry Sample Processing Form (IHC-SPF-01). If you require more than 10 slides to be stained, then please use additional copies of this form.
To ensure that your tissue and antibody samples are processed properly, it is necessary to complete either the Confidential (IHC-CSDF-01) or Non-Confidential (IHC-NSDF-01) Sample Description Form as appropriate. If you are supplying the probing antibody, then please also complete the Client-Supplied Antibody Description Form (IHC-CADF-1). These and any other relevant forms should be included with the samples sent to Kinexus for analyses. More information about the various forms that need to be completed is provided in our customer information package for these services.
Basic Shipping Information
Kinexus Bioinformatics Corporation
Suite 1, 8755 Ash Street
Vancouver, B.C. Canada V6P 6T3
Telephone: (604) 323-2547
Facsimile: (604) 323-2548
Please ensure 3 copies of a signed commercial invoice accompany your shipment which specifies your samples are non hazardous and non infectious. Three different commercial invoice templates are supplied in our customer information package for these services, and these should be used depending on whether the samples are being sent as paraffin-embedded, sectioned on glass slides or frozen tissues. Since the samples are not for resale, the value of your shipment should be priced at approximately $1.00 per sample. It is also highly recommended that customers email their Federal Express airway bill number and the date of departure to firstname.lastname@example.org so we can track your shipment in transit and ensure it arrives in a timely manner. We will send a confirmation email once your shipment arrives at our facility.