KPSS Protein Phosphatase Profiling
Kinetworks™ Phosphatase Screen 1.2
Protein phosphatases play a critical role in the regulation of signal transduction pathways. Target proteins can be phosphorylated at specific serine, threonine and/or tyrosine residues by protein kinases, and the phosphate group is removed by the action of specific protein phosphatases. Protein phosphatases can be divided into 3 broad categories – those that target phosphorylated serine/threonine (PSTP) and tyrosine (PYP), and dual specificity (PSTYP).
Many serine/threonine phosphatases are holoenzymes, composed of regulatory, catalytic and variable subunits. Dual specificity phosphatases dephosphorylate proteins on phosphotyrosine, phosphoserine and/or phosphothreonine residues. The protein-tyrosine phosphatase category contains many transmembrane proteins that participate in cell to cell communication and in signal transduction within the cell.
Features of the Kinetworks™ Phosphatase Screen 1.2 include:
- Simultaneous identification and activation assessment of a network of 27 phosphatase proteins.
- Use of the best of over 27 phosphatase antibodies pre-tested and validated for specificity and efficacy within the Kinetworks™ assay systems.
- Qualitative results with TIFF image of the immunoblot.
- Quantitative results with measurement of the intensity of the immunoblot signals over a thousand-fold range.
- Bar graphical representation of immunoblotting results.